This preprint has been published elsewhere.
DOI of the published preprint http://doi.org/10.1016/j.jviromet.2020.113888
Preprint / Version 1

Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19

##article.authors##

  • Gubio S. Campos Institute of Health Sciences, Federal University of Bahia, Salvador-BA, Brazil https://orcid.org/0000-0002-1700-2462
    • Silvia I. Sardi Institute of Health Sciences, Federal University of Bahia, Salvador-BA, Brazil https://orcid.org/0000-0002-2002-8436
      • Melissa B. Falcao Núcleo de Pesquisa e Extensão em Vigilância da Saúde, Universidade Estadual de Feira de Santana, Feira de Santana-BA, Brazil
        • Emilia M. M. A. Belitardo Institute of Health Sciences, Federal University of Bahia, Salvador-BA, Brazil https://orcid.org/0000-0003-0818-1582
          • Danilo J. P. G. Rocha Institute of Health Sciences, Federal University of Bahia, Salvador-BA, Brazil https://orcid.org/0000-0002-2160-2153
            • Carolina A. Rolo Institute of Health Sciences, Federal University of Bahia, Salvador-BA, Brazil https://orcid.org/0000-0001-8886-6340
              • Aline D. Menezes Institute of Health Sciences, Federal University of Bahia, Salvador-BA, Brazil https://orcid.org/0000-0002-1416-2874
                • Carina S. Pinheiro Institute of Health Sciences, Federal University of Bahia, Salvador-BA, Brazil https://orcid.org/0000-0001-5623-1308
                  • Rejane H. Carvalho Institute of Health Sciences, Federal University of Bahia, Salvador-BA, Brazil
                    • João P. P. Almeida Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte-MG, Brazil https://orcid.org/0000-0001-7532-8420
                      • Eric R. G. R. Aguiar Department of Biological Sciences, Center of Biotechnology and Genetics, State University of Santa Cruz (UESC), Ilhéus-BA, Brazil https://orcid.org/0000-0002-8143-5756
                        • Luis G. C. Pacheco Institute of Health Sciences, Federal University of Bahia, Salvador-BA, Brazil https://orcid.org/0000-0003-2500-0800

                          DOI:

                          https://doi.org/10.1590/SciELOPreprints.239

                          Keywords:

                          Covid-19, SARS-CoV-2, next-generation sequencing, metatranscriptomics

                          Abstract

                          Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.

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                          Posted

                          04/29/2020

                          How to Cite

                          Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19. (2020). In SciELO Preprints. https://doi.org/10.1590/SciELOPreprints.239

                          Section

                          Biological Sciences

                          Copyright (c) 2020 Gubio S. Campos, Silvia I. Sardi, Melissa B. Falcao, Emilia M. M. A. Belitardo, Danilo J. P. G. Rocha, Carolina A. Rolo, Aline D. Menezes, Carina Pinheiro, Rejane H. Carvalho, João P. P. Almeida, Eric R. G. R. Aguiar, Luis G. C. Pacheco

                          Creative Commons License

                          This work is licensed under a Creative Commons Attribution 4.0 International License.

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