Preprint / Version 1

EVALUATION OF THE IMMUNOLOCALIZATION OF THE PHOSPHATIDYL INOSITOL-3 RECEPTOR (IP3R) IN BASAL CELL AND SQUAMOUS CELL SKIN CARCINOMAS

##article.authors##

  • Cristiane Regina Gruber Faculdade Evangélica Mackenzie do Paraná, Curitiba, PR, Brasil
  • Thelma Larocca Skare Faculdade Evangélica Mackenzie do Paraná
  • Allan Fernando Giovanini Faculdade Evangélica Mackenzie do Paraná, Curitiba, PR, Brasil
  • Marcos Fabiano Sigwalt Faculdade Evangélica Mackenzie do Paraná, Curitiba, PR, Brasil
  • Graziela J. Crescente Rastelli Serviço de Dermatolgia do Hospital Universitário Evangélico Mackenzie, Curitiba, PR, Brasil
  • Fernando Issamu Tabushi Faculdade Evangélica Mackenzie do Paraná, Curitiba, PR, Brasil https://orcid.org/0000-0002-3150-2164

DOI:

https://doi.org/10.1590/SciELOPreprints.7843

Keywords:

Squamous cell carcinoma, Channelopathy, IP3R, Immunohistochemistry

Abstract

Introduction: Non-melanoma skin neoplasms (basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) form the most common group of all types of neoplasms. Although cancer is not yet being classified as a channelopathy, it has been suggested that pumps and ionic channels contribute to its progression by affecting autophagy, which could become an important therapeutic target in this context.

Objective: To evaluate the immunohistochemical expression of IP3R in both skin tumors.

Method: Immunohistochemistry was performed on 60 slides of non-melanoma cancer using primary anti-IP3R antibodies, verifying their presence and quantifying

Results: For the first time, IP3R immunolocalization was identified in non-melanoma skin neoplasms, being evident above 90% of neoplastic cells was observed in all slides studied, regardless of the histological pattern, invasion and other tumor characteristics. Unlike what was seen in the internal control of normal skin, in which there was immunolocalization of IP3R in basal cell, in tumors, immunohistochemical expression occurred throughout the entire body of the neoplasm.

Conclusion: There was immunolocalization of IP3R in tumor cells in both BCC and SCC. It was not possible to establish a correlation between tumor characteristics and IP3R expression, as immunostaining was similar in all analyzed samples. Despite this, IP3R to be associated with the pathophysiology of non-melanoma skin cancer, but its expression does not seem to be associated with tumor aggressiveness.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Posted

01/05/2024

How to Cite

Gruber, C. R., Skare, T. L., Giovanini, A. F., Sigwalt, M. F., Rastelli, G. J. C., & Tabushi, F. I. (2024). EVALUATION OF THE IMMUNOLOCALIZATION OF THE PHOSPHATIDYL INOSITOL-3 RECEPTOR (IP3R) IN BASAL CELL AND SQUAMOUS CELL SKIN CARCINOMAS. In SciELO Preprints. https://doi.org/10.1590/SciELOPreprints.7843

Section

Health Sciences

Plaudit

Data statement

  • The research data is contained in the manuscript