AVALIAÇÃO DA IMUNOLOCALIZAÇÃO DO RECEPTOR DO FOSFATIDIL INOSITOL-3 (IP3R) EM CARCINOMAS BASOCELULAR E DE CÉLULAS ESCAMOSAS DE PELE

Cristiane Regina Gruber; Thelma Larocca Skare; Allan Fernando Giovanini; Marcos Fabiano Sigwalt; Graziela J. Crescente Rastelli; Fernando Issamu Tabushi

doi:10.1590/SciELOPreprints.7843

##article.authors##

Cristiane Regina Gruber Faculdade Evangélica Mackenzie do Paraná, Curitiba, PR, Brasil
Thelma Larocca Skare Faculdade Evangélica Mackenzie do Paraná
Allan Fernando Giovanini Faculdade Evangélica Mackenzie do Paraná, Curitiba, PR, Brasil
Marcos Fabiano Sigwalt Faculdade Evangélica Mackenzie do Paraná, Curitiba, PR, Brasil
Graziela J. Crescente Rastelli Serviço de Dermatolgia do Hospital Universitário Evangélico Mackenzie, Curitiba, PR, Brasil
Fernando Issamu Tabushi Faculdade Evangélica Mackenzie do Paraná, Curitiba, PR, Brasil https://orcid.org/0000-0002-3150-2164

DOI:

https://doi.org/10.1590/SciELOPreprints.7843

Keywords:

Squamous cell carcinoma, Channelopathy, IP3R, Immunohistochemistry

Abstract

Introduction: Non-melanoma skin neoplasms (basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) form the most common group of all types of neoplasms. Although cancer is not yet being classified as a channelopathy, it has been suggested that pumps and ionic channels contribute to its progression by affecting autophagy, which could become an important therapeutic target in this context.

Objective: To evaluate the immunohistochemical expression of IP3R in both skin tumors.

Method: Immunohistochemistry was performed on 60 slides of non-melanoma cancer using primary anti-IP3R antibodies, verifying their presence and quantifying

Results: For the first time, IP3R immunolocalization was identified in non-melanoma skin neoplasms, being evident above 90% of neoplastic cells was observed in all slides studied, regardless of the histological pattern, invasion and other tumor characteristics. Unlike what was seen in the internal control of normal skin, in which there was immunolocalization of IP3R in basal cell, in tumors, immunohistochemical expression occurred throughout the entire body of the neoplasm.

Conclusion: There was immunolocalization of IP3R in tumor cells in both BCC and SCC. It was not possible to establish a correlation between tumor characteristics and IP3R expression, as immunostaining was similar in all analyzed samples. Despite this, IP3R to be associated with the pathophysiology of non-melanoma skin cancer, but its expression does not seem to be associated with tumor aggressiveness.