Implementation and preliminary evaluation of the Tumor Microenvironment of Co-Cultive HTB-125/HTB-126 in response to treatment with Potassium Ribose Ascorbate or Potassium Ribosate

Abstract

The implantation of a malignant cell or a metastatic colony is an indisputable indication of the combined response of tumor cells and healthy cells, and is the final consequence of an active, continuous, complex, and multi-step process called the metastatic cascade. One of the important points is to stop this process before it strengthens and modifies the extracellular matrix. This first mechanism involves the adhesion factors or binding glycoproteins that are mediated by receptors of tumor cells that if inhibited would prevent the metastatic process. Understanding the mechanisms of communication between triple-negative breast tumor cells and healthy breast cells is essential to design effective therapeutic strategies taking into account the microenvironment of the tumor. In these trials a co-culture composed of Hs 578Bst (HTB-125 healthy epithelial) and Hs 578T (HTB-126 triple negative tumor epithelial, ATCC® No. TCP-1002TM, derived from breast carcinoma) was implemented, in this co-culture it has been observed that, although the factors secreted by one cell line do not have a marked negative effect on the proliferation of the other cell line, it does influence the development of the angiogenic process of endothelial cells.  This model of cells in active colonization that we have designed, has allowed us to show that the response of Potassium Ribose Ascorbate and of the Potassium Ribosate on it was the inhibition of the advance of the tumor microenvironment and the decrease of the tumor cells that can migrate under conditions of latency.