Biofungicidal effect of two ethanolic extracts based on grapefruit (Citrus × Paradisi) and Tagete (Tagetes erecta L.) with Ruda (Ruta graveolens L.) on gray mold (Botrytis cinerea) under in vitro conditions

Abstract

In view of the increasing pollution generated by the use of synthetic pesticides and due to the increase in the resistance of fungicides, has led to the development of new alternatives for the control of Botrytis cinerea, a fungus of great agricultural importance that generates significant economic losses. One of these methods is the use of natural extracts in order to generate the least damage environmental, in addition its application constitutes an important alternative in fruits for fresh consumption. Under this premise, the objective of this work was determine the in vitro antifungal activity of two ethanolic extracts of C. Paradisi and T. erecta L. with R. graveolens L. evaluated in PDA medium with three repetitions and four treatments, the two extracts (CR and CP), a controller biological antagonist Trichoderma Harzianum (CB), using as a control the commercial chemical fungicide Captan (CQx). The results obtained showed that the variables to be measured: percentage of inhibition, antagonism of the controller biological and percentage of mycelial growth inhibition have an effectiveness inhibitory of CR (22.9%) compared to CQx (25.9%) and CP was not efficient in the control in vitro of cinerea. On the other hand, the type of antagonism of Trichoderma Harzianum was not as expected for this phytopathogen either.