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USE OF DECELLULARIZED HUMAN AMNIOTIC MEMBRANE IN INTESTINAL ANASTOMOSES: A STUDY IN RATS TREATED WITH 5-FLUOROURACIL

##article.authors##

  • Daniel Dantas Ferrarin Faculdade Evangélica Mackenzie do Paraná
  • Nicolau Gregori Czeczko Faculdade Evangélica Mackenzie do Paraná
  • Luiz Fernando Kubrusly Faculdade Evangélica Mackenzie do Paraná
  • Osvaldo Malafaia Colégio Brasileiro de Cirurgia Digestiva https://orcid.org/0000-0002-1829-7071
  • Eros Luiz de Sousa Faculdade Evangélica Mackenzie do Paraná
  • João Carlos Domingues Repka Hospital e Maternidade Angelina Caron
  • Pedro Henrique Lambach Caron Faculdade Evangélica Mackenzie do Paraná
  • Carlos José Franco de Souza Hospital e Maternidade Angelina Caron

DOI:

https://doi.org/10.1590/SciELOPreprints.4505

Keywords:

Amniotic membrane, Anastomosis, Antitumoral chemotherapy

Abstract

Introduction: currently, the most relevant applications of perinatal derivatives are in wound healing, for example, the use of amniotic membrane (AM) as a biomaterials source for use in different healing processes. The anastomotic healing compromised by antimetabolic drugs, such as 5-fluorouracil (5-FU), is a potential target of AM. Objective: To evaluate the healing effects of the AM use in rats treated with 5-FU at a dose of 20 mg/kg on the 7th postoperative day, regarding the parameters percentage of type I collagen (mature), cell viability, microvascular density and granulation tissue formation. Method: Thirty-two Wistar rats were submitted to colotomy and colorraphy, separated in 4 groups with 8t rats each and received, by 7 days, different treatments intraperitoneally, once daily from the operation day until sacrifice: 1- saline solution (C), 2- 20 mg/kg 5-FU, 3- 20 mg/kg 5-FU and 4- MA, and MA. Results: Tthe treatment with 5-FU (20 mg/kg – 7 days) induced adverse effects in the anastomotic healing process evidenced by the decrease of type I collagen (mature), cell viability, percentage microvascular density, granulation tissue formation (leukocyte-fibrin crust and angio-fibroblastic proliferation). The use of AM, under these conditions, induced an improvement in type I collagen (mature) percentage. Conclusion: The treatment with 5-FU induced adverse effects on the healing anastomotic process, and the use of AM, under these conditions, induced improvement in type I (mature) collagen percentage.

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Posted

07/27/2022

How to Cite

Ferrarin, D. D., Czeczko , N. G., Kubrusly , L. F., Malafaia, O., Sousa, E. L. de, Repka, J. C. D., Caron, P. H. L., & Souza , C. J. F. de. (2022). USE OF DECELLULARIZED HUMAN AMNIOTIC MEMBRANE IN INTESTINAL ANASTOMOSES: A STUDY IN RATS TREATED WITH 5-FLUOROURACIL. In SciELO Preprints. https://doi.org/10.1590/SciELOPreprints.4505

Section

Health Sciences
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