Clinical parameters of individuals with periodontitis and Diabetes Mellitus reveals signatures of the minority microbiota in saliva

Débora Côrtes; Jéssica de Cena; Letícia Marconatto; Maria do Carmo Guimarães; Daniela Grisi; Laudimar de Oliveira; Loise Salles; Luiz Gustavo Borges; Nailê Damé-Teixeira

doi:10.1590/SciELOPreprints.3093

article.authors698aa2ae4d8f0

Débora Côrtes Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF, Brazil https://orcid.org/0000-0003-0133-6836
Jéssica de Cena Department of Dentistry, School of Health Sciences, University of Brasília, Brasília, DF, Brazil
Letícia Marconatto Institute of Petroleum and Natural Resources, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-5578-5920
Maria do Carmo Guimarães Department of Dentistry, School of Health Sciences, University of Brasília, Brasília, DF, Brazil
Daniela Grisi Department of Dentistry, School of Health Sciences, University of Brasília, Brasília, DF, Brazil
Laudimar de Oliveira Department of Dentistry, School of Health Sciences, University of Brasília, Brasília, DF, Brazil
Loise Salles Department of Dentistry, School of Health Sciences, University of Brasília, Brasília, DF, Brazil https://orcid.org/0000-0003-1061-7963
Luiz Gustavo Borges Institute of Petroleum and Natural Resources, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil
Nailê Damé-Teixeira Department of Dentistry, School of Health Sciences, University of Brasília, Brasília, DF, Brazil https://orcid.org/0000-0002-0490-0036

DOI:

https://doi.org/10.1590/SciELOPreprints.3093

Palavras-chave:

periodontal disease, type 2 diabetes, low abundance microorganisms

Resumo

Aims: This pilot study aimed to describe the majority and minority microbiota of saliva from individuals with advanced periodontitis, with or without type 2 diabetes mellitus (T2D), correlating the relative abundance of microorganisms with clinical parameters.

Material/Methods: Six individuals diagnosed with periodontitis were included and classified according to their diagnosis of T2D. Salivary pH, number of teeth with active caries lesions, number of remaining teeth, periodontal and glycemic parameters were evaluated. V4 region amplicons of the 16S rRNA from salivary DNA were sequenced at Ion PGM. Amplicon sequence variants (ASVs) were compared according to the clinical parameters.

Results: Correlation showed eight low-abundant bacteria significantly correlated with co-variables, either positively or negatively. The periodontitis-associated bacteria followed the increasing pH and number of remaining teeth.

Conclusions: This survey provided potential minority microbiota correlations with clinical parameters, such as number of remaining teeth, FBG, and salivary pH. The ubiquity of some low abundant microorganisms in individuals with advanced periodontitis, exposed or not to type 2 DM, can reveal microbial signatures not yet explored.