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A new milk-clotting enzyme from noni seeds (Morinda citrifolia L)

##article.authors##

  • Julyanne Dantas de Oliveira Federal University of Rio de Janeiro
  • César Luis Siqueira Junior Federal University of Rio de Janeiro https://orcid.org/0000-0001-8668-2222

DOI:

https://doi.org/10.1590/2236-8906-56/2021

Keywords:

milk clotting, proteolytic activity, protease, seeds

Abstract

They are found in all living organisms and in plants has received more attention because of their potential involvement in various industrial processes. Noni plant (Morinda citrifolia L.), belongs to the family Rubiaceae, of southwestern Asia origin. The juice made with the fruit is widely used as phytotherapic agent and in combating a range of diseases, while seeds are usually discarded. The objective of this work was to seek active milk clotting proteases in noni seeds. The crude extract (CE) protein was obtained from seeds sprayed and mixed with tris-HCl 50mM buffer (pH 6.0). The results demonstrated the presence of proteases with milk clotting properties in noni seeds, which a high milk clotting activity from 3,891 U/mL to 65°C.  This value indicates that 1mL of extract is able to coagulate 3.8 liters of milk in 40 minutes under specific conditions. This property indicates the potential of noni seeds as a natural source of proteolytic enzymes with biotechnological potential for application in the cheese making industry.

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Posted

08/01/2022

How to Cite

Oliveira, J. D. de, & Siqueira Junior, C. L. (2022). A new milk-clotting enzyme from noni seeds (Morinda citrifolia L). In SciELO Preprints. https://doi.org/10.1590/2236-8906-56/2021

Section

Biological Sciences

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